PLK1 Rabbit Polyclonal Antibody (HA500281)
Catalog# HA500281
Rabbit Polyclonal to PLK1
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of PLK1 on different lysates with Rabbit anti-PLK1 antibody (HA500281) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
