ROCK1 Rabbit Polyclonal Antibody (ER40122)
Catalog# ER40122
Rabbit Polyclonal to ROCK1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: C6 cell lysate
Lane 5: L6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: C6 cell lysate
Lane 5: L6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.