RCL Recombinant Rabbit Monoclonal Antibody [JE59-29] (HA720027)
Catalog# HA720027
Rabbit Monoclonal to RCL
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of RCL on different lysates with Rabbit anti-RCL antibody (HA720027) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RCL KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720027) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-RCL KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720027) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.