K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody [JM09-67] (ET1703-07)

UNIPROT #

P0CG47

PROTEIN NAME

K63-linkage Specific Ubiquitin

SYNONYMS

Epididymis secretory protein Li 50 antibody;FLJ25987 antibody;HEL S 50 antibody;MGC8385 antibody;Polyubiquitin B antibody;RPS 27A antibody;RPS27A antibody;UBA 52 antibody;UBA 80 antibody;UBA52 antibody;UBA80 antibody;UBB antibody;UBB_HUMAN antibody;UBC antibody;UBCEP 1 antibody;UBCEP 2 antibody;UBCEP1 antibody;UBCEP2 antibody;Ubiquitin antibody;Ubiquitin B antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin family.

POST-TRANSLATIONAL MODIFICATION

[Ubiquitin]: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.; [Ubiquitin]: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms. Four genes in the human genome code for ubiquitin: UBB, UBC, UBA52 and RPS27A. The addition of ubiquitin to a substrate protein is called ubiquitylation (or, alternatively, ubiquitination or ubiquitinylation). Ubiquitylation affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitylation involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. Only polyubiquitylation on defined lysines, mostly on K48 and K29, is related to degradation by the proteasome (referred to as the "molecular kiss of death"), while other polyubiquitylations (e.g. on K63, K11, K6 and M1) and monoubiquitylations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.