Rb Recombinant Rabbit Monoclonal Antibody [SY63-03] (ET1607-9)

UNIPROT #

P06400

PROTEIN NAME

Rb

SYNONYMS

Exon 17 tumor GOS561 substitution mutation causes premature stop antibody;GOS563 exon 17 substitution mutation causes premature stop antibody;OSRC antibody;Osteosarcoma antibody;p105-Rb antibody;P105RB antibody;PP105 antibody;pp110 antibody;PPP1R130 antibody;pRb antibody;Prepro retinoblastoma associated protein antibody;Protein phosphatase 1 regulatory subunit 130 antibody;Rb antibody;RB transcriptional corepressor 1 antibody;RB_HUMAN antibody;RB1 antibody;RB1 gene antibody;Retinoblastoma 1 antibody;Retinoblastoma suspectibility protein antibody;Retinoblastoma-associated protein antibody

SEQUENCE SIMILARITIES

Belongs to the retinoblastoma protein (RB) family.

TISSUE SPECIFICITY

Expressed in the retina. Expressed in foreskin keratinocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).; N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.; Acetylated during keratinocyte differentiation. Acetylation at Lys-873 and Lys-874 regulates subcellular localization. Can be deacetylated by SIRT1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated Rb, RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, inactivating it, and the cell cycle is allowed to progress. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomavirus, bind and inactivate pRb, this can lead to cancer. The RB gene may have been responsible for the evolution of multicellularity in several lineages of life including animals.