AFP Recombinant Rabbit Monoclonal Antibody [PSH11-75] (HA723371)
Catalog# HA723371
Rabbit Monoclonal to AFP
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of AFP on different lysates with Rabbit anti-AFP antibody (HA723371) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Mouse placenta tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Mouse placenta tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.