SHP1 Recombinant Rabbit Monoclonal Antibody [SR41-02] (ET1602-19)
Catalog# ET1602-19
Rabbit Monoclonal to SHP1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: RAW264.7 cell lysate (15 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: Rat thymus tissue lysate (20 µg/Lane)
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 24 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-19) at 1/5,000dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
SHP1 Recombinant Rabbit Monoclonal Antibody [SR41-02] (ET1602-19)
Immunogen
Synthetic peptide within human shp1 aa 537-595 / 595.
Host
Rabbit
Positive Control
Jurkat cell lysate, RAW264.7 cell lysate, Mouse spleen tissue lysate, Rat thymus tissue lysate, Jurkat, RAW264.7, human tonsil tissue, mouse spleen tissue, rat brain tissue, rat spleen tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR41-02
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:1,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
SHP1
SYNONYMS
70Z-SHP antibody;EC 3.1.3.48 antibody;HCP antibody;HCPH antibody;Hematopoietic cell phosphatase antibody;Hematopoietic cell protein tyrosine phosphatase antibody;Hematopoietic cell protein-tyrosine phosphatase antibody;HPTP1C antibody;Protein tyrosine phosphatase 1C antibody;Protein tyrosine phosphatase non receptor type 6 antibody;Protein tyrosine phosphatase SHP1 antibody;Protein-tyrosine phosphatase 1C antibody;protein-tyrosine phosphatase SHP 1 antibody;Protein-tyrosine phosphatase SHP-1 antibody;PTN6_HUMAN antibody;PTP 1C antibody;PTP-1C antibody;PTP1C antibody;Ptpn6 antibody;SH PTP 1 antibody;SH PTP1 antibody;SH-PTP1 antibody;SHP 1 antibody;SHP 1L antibody;SHP1 antibody;SHP1L antibody;tyrosine protein phosphatase non receptor type 6 antibody;Tyrosine-protein phosphatase non-receptor type 6 antibody
SEQUENCE SIMILARITIES
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
TISSUE SPECIFICITY
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Tyrosine-protein phosphatase non-receptor type 6, also known as Src homology region 2 domain-containing phosphatase-1 (SHP-1), is an enzyme that in humans is encoded by the PTPN6 gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling, (e.g., the LYN-CD22-SHP-1 pathway). Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
CITATIONS
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Bin Li,Gongyou Chen
New insights into virulence mechanisms of rice pathogen Acidovorax avenae subsp. avenae strain RS-1 following exposure to ß-lactam antibiotics
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WB
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Escherichia coli
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Citation
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