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Human IFN-α enzyme-linked immunoassay kit(EH0085)

Price: Regular price $499.00

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Catalog# EH0085

Assay type Sandwich ELISA

Range n.d.-0.9

Sensitivity 0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product_detail

pg/ml O.D. Average Corrected
0.00 0.0187 0.0181 0.0184
0.41 0.0407 0.0408 0.0408 0.0224
1.23 0.0767 0.0753 0.0760 0.0576
3.70 0.1796 0.1758 0.1777 0.1593
11.11 0.4858 0.4840 0.4849 0.4665
33.33 1.2260 1.2340 1.2300 1.2116
100.00 2.5610 2.5950 2.5780 2.5596
300.00 4.2605 4.2605 4.2605 4.2421

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)11.4 51.2155.612.057.0175
Standard Deviation0.53.89.80.71.84.2
Coefficient of Variation(%)4.47.36.35.33.12.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-0.931.30.21

Sensitivity

0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)

Range

n.d.-0.9

Recovery

The spike recovery was evaluated by spiking 3 levels of human IFN-alpha into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 128% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IFN-α enzyme-linked immunoassay kit(EH0085)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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