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Human IFN-gamma enzyme-linked immunoassay kit (EH0039)

Price: Regular price $499.00

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Catalog# EH0039

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.36 pg/ml (50 μl)；0.85 pg/ml (10 μl)

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01170.01210.0119
1.370.02500.02820.02660.0147
4.120.04620.04890.04760.0357
12.350.12480.12400.12440.1125
37.040.33990.33930.33960.3277
111.110.92010.89540.90780.8959
333.332.01601.99102.00351.9916
1000.003.69403.67303.68353.6716

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)17.298.2273.318.991.8240.1
Standard Deviation0.536.80.82.75.2
Coefficient of Variation(%)2.83.22.54.232.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-446.289293.56

Sensitivity

0.36 pg/ml (50 μl)；0.85 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IFN gamma into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 89% to 113% with an overall mean recovery of 105%

Specifications

Product Type

ELISA Kit

Product Name

Human IFN-gamma enzyme-linked immunoassay kit (EH0039)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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