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Human Total HSP60 enzyme-linked immunoassay kit (EH0060)

Price: Regular price $499.00

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Catalog# EH0060

Assay type Sandwich ELISA

Standard curve range 4.10~1000 pg/ml

Sensitivity 3.22 pg/ml (50 μl)；4.45 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03100.03270.0319
4.100.04360.04620.04490.0131
10.240.06780.06910.06850.0366
25.600.11960.12360.12160.0898
64.000.25350.26210.25780.2260
160.000.55220.57830.56530.5334
400.001.24601.27001.25801.2262
1000.002.58402.55402.56902.5372

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)26.699.0277.027.8101.2284.9
Standard Deviation2.35.621.71.85.314.5
Coefficient of Variation(%)8.55.67.86.35.25.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-20.90508.98

Sensitivity

3.22 pg/ml (50 μl)；4.45 pg/ml (10 μl)

Standard curve range

4.10~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Total HSP60 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 66% to 73% with an overall mean recovery of 69%.

Specifications

Product Type

ELISA Kit

Product Name

Human Total HSP60 enzyme-linked immunoassay kit (EH0060)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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