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Mouse IFN-β enzyme-linked immunoassay kit (EM0040)

Price: Regular price $499.00

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Catalog# EM0040

Assay type Sandwich ELISA

Standard curve range 46.88~3000 pg/ml

Sensitivity 7.69 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.04110.03650.0388
46.880.06650.06000.06330.0245
93.750.09450.08930.09190.0531
187.500.15430.15010.15220.1134
375.000.28480.28500.28490.2461
750.000.58980.58640.58810.5493
1500.001.38201.27901.33051.2917
3000.003.27203.20603.23903.2002

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)59.5302.91001.773.4302.3911.3
Standard Deviation4.222.456.95.714.552.5
Coefficient of Variation(%)7.07.45.77.74.85.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-31.133.331.13

Sensitivity

7.69 pg/ml (10 μl)

Standard curve range

46.88~3000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IFN beta into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 82% to 106% with an overall mean recovery of 96%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IFN-β enzyme-linked immunoassay kit (EM0040)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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