KAP1 Recombinant Rabbit Monoclonal Antibody [SD081-05] (ET1612-55)

UNIPROT #

Q13263

PROTEIN NAME

KAP1

SYNONYMS

E3 SUMO protein ligase TRIM28 antibody;E3 SUMO-protein ligase TRIM28 antibody;FLJ29029 antibody;KAP 1 antibody;KAP-1 antibody;KRAB associated protein 1 antibody;KRAB interacting protein 1 antibody;KRAB-associated protein 1 antibody;KRAB-interacting protein 1 antibody;KRIP 1 antibody;KRIP-1 antibody;KRIP1 antibody;Nuclear corepressor KAP 1 antibody;Nuclear corepressor KAP-1 antibody;RING finger protein 96 antibody;RNF96 antibody;TF1B antibody;TIF1 beta antibody;TIF1-beta antibody;TIF1B antibody;TIF1B_HUMAN antibody;Transcription intermediary factor 1 beta antibody;Transcription intermediary factor 1-beta antibody;Trim28 antibody;Tripartite motif containing 28 antibody;tripartite motif containing protein 28 antibody;Tripartite motif-containing protein 28 antibody

SEQUENCE SIMILARITIES

Belongs to the TRIM/RBCC family.

TISSUE SPECIFICITY

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

POST-TRANSLATIONAL MODIFICATION

ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.; Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.; Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2.; Citrullinated by PADI4.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein.