ERK2 Recombinant Rabbit Monoclonal Antibody [SZ25-01] (ET1603-23)

UNIPROT #

P28482

PROTEIN NAME

ERK2

SYNONYMS

ERK 2 antibody;ERK antibody;ERK-2 antibody;ERT1 antibody;Extracellular Signal Regulated Kinase 2 antibody;Extracellular signal-regulated kinase 2 antibody;MAP kinase 1 antibody;MAP kinase 2 antibody;MAP kinase isoform p42 antibody;MAPK 1 antibody;MAPK 2 antibody;Mapk1 antibody;MAPK2 antibody;Mitogen-activated protein kinase 1 antibody;Mitogen-activated protein kinase 2 antibody;MK01_HUMAN antibody;P38 antibody;P40 antibody;P41 antibody;p42-MAPK antibody;P42MAPK antibody;PRKM1 antibody;PRKM2 antibody;protein kinase, mitogen-activated, 1 antibody;protein kinase, mitogen-activated, 2 antibody;protein tyrosine kinase ERK2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. Dephosphorylated by DUSP1 at Thr-185 and Tyr-187.; ISGylated.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cytoskeleton, Membrane, Cell junction.

FUNCTION

Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein.