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PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68] (ET1608-56)

Price: Regular price $385.00

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Catalog# ET1608-56

Rabbit Monoclonal to PARP1

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

Select your chosen country/region

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68] (ET1608-56)

Immunogen

Synthetic peptide within n-terminal human parp1.

Host

Rabbit

Positive Control

HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, HeLa whole cell lysate, HeLa treated with 1μM staurosporine for 3 hours whole cell lysate, HeLa, human breast cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU03-68

RRID

AB_3069814

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IF-Cell

  • 1:500

  • IF-Tissue

  • 1:50-1:800

  • IHC-P

  • 1:4,000

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 113 kDa

Isotype

IgG

TARGET

UNIPROT #

P09874

PROTEIN NAME

PARP1

SYNONYMS

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody;ADP ribosyltransferase diphtheria toxin like 1 antibody;ADP ribosyltransferase NAD(+) antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;ARTD1 antibody;msPARP antibody;NAD(+) ADP ribosyltransferase 1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;pADPRT 1 antibody;pADPRT1 antibody;PARP 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly (ADP ribose) polymerase 1 antibody;poly (ADP ribose) polymerase family, member 1 antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly(ADP ribose) polymerase antibody;poly(ADP ribose) synthetase antibody;poly(ADP ribosyl)transferase antibody;Poly[ADP ribose] synthetase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION

Nucleus, nucleolus, chromosome.

FUNCTION

The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.

CITATIONS

  • Jiang Huangyu,et al

    Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway

  • Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D.

    BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency

  • Chao Shen

    Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signalling

  • Dr Xia Liu,Professor Jianchao Wang

    Silencing RRM2 inhibits multiple myeloma by targeting the Wnt/β-catenin signaling pathway

  • Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G.

    N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma

  • Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway

  • Xiang, X., Tian, Y., Hu, J., Xiong, R., Bautista, M., Deng, L., Yue, Q., Li, Y., Kuang, W., Li, J., Liu, K., Yu, C., & Feng, G.

    Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical pharmacology, 186, 114475.

  • Liu, X., Cui, S., Dan, C., Li, W., Xie, H., Li, C., & Shi, L.

    Phellinus baumii Polyphenol: A Potential Therapeutic Candidate against Lung Cancer Cells

  • Fang Xiao,et al

    Chromosome instability functions as a potential therapeutic reference by enhancing chemosensitivity to BCL-XL inhibitors in colorectal carcinoma

  • Hu, J., Cao, J., Jin, R., Zhang, B., Topatana, W., Juengpanich, S., Li, S., Chen, T., Lu, Z., Cai, X., & Chen, M.

    Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

  • Apatinib Inhibits Bladder Cancer through Suppression of the VEGFR2-PI3K-AKT Signaling Pathway as Revealed by Network Pharmacology and in vitro Experimental Verification

  • Elucidation of the anti-colon cancer mechanism of Phellinus baumii polyphenol by an integrative approach of network pharmacology and experimental verification

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