CENPB Mouse Monoclonal Antibody [A5F6] (HA600071)
Catalog# HA600071
Mouse Monoclonal to CENPB
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of CENPB on different lysates with Mouse anti-CENPB antibody (HA600071) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: Daudi cell lysate
Lane 3: 3T3-L1 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600071) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: Daudi cell lysate
Lane 3: 3T3-L1 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600071) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.