Phospho-SMC1 (S957) Recombinant Rabbit Monoclonal Antibody [SC65-03] (ET1610-55)
Catalog# ET1610-55
Rabbit Monoclonal to Phospho-SMC1 (S957)
Tested for
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WB
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IHC-P
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IF-Cell
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-SMC1 (S957) on different lysates with Rabbit anti-Phospho-SMC1 (S957) antibody (ET1610-55) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with UV then recovery 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 144 kDa
Observed band size: 150 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with UV then recovery 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 144 kDa
Observed band size: 150 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.