EDD Recombinant Rabbit Monoclonal Antibody [JE38-53] (HA721646)
Catalog# HA721646
Rabbit Monoclonal to EDD
Tested for
-
WB
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IHC-P
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IF-Cell
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of EDD on different lysates with Rabbit anti-EDD antibody (HA721646) at 1/1,000 dilution.
Lane 1: Neuro-2a cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Jurkat cell lysate
Lane 4: A549 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: LNCaP cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 309 kDa
Observed band size: 309 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: Neuro-2a cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Jurkat cell lysate
Lane 4: A549 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: LNCaP cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 309 kDa
Observed band size: 309 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.