UBE4B Recombinant Rabbit Monoclonal Antibody [JE55-76] (ET7111-11)
Catalog# ET7111-11
Rabbit Monoclonal to UBE4B
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of UBE4B on different lysates with Rabbit anti-UBE4B antibody (ET7111-11) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Raji cell lysate (10 µg/Lane)
Lane 3: RAW264.7 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 146 kDa
Observed band size: 146 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Raji cell lysate (10 µg/Lane)
Lane 3: RAW264.7 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 146 kDa
Observed band size: 146 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
