HDAC3 Mouse Monoclonal Antibody [B2-G9] (M1511-3)
Catalog# M1511-3
Mouse Monoclonal to HDAC3
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Western blot analysis of HDAC3 on different lysates with Mouse anti-HDAC3 antibody (M1511-3) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-HDAC3 KD cell lysate (10 µg/Lane)
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-3) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-HDAC3 KD cell lysate (10 µg/Lane)
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-3) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.