PARP1 Mouse Monoclonal Antibody [A0-D11] (EM1701-16)

UNIPROT #

P09874

PROTEIN NAME

PARP1

SYNONYMS

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody;ADP ribosyltransferase antibody;ADP ribosyltransferase diphtheria toxin like 1 antibody;ADP ribosyltransferase NAD(+) antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;ARTD1 antibody;msPARP antibody;NAD(+) ADP ribosyltransferase 1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;pADPRT 1 antibody;pADPRT1 antibody;PARP 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly (ADP ribose) polymerase 1 antibody;poly (ADP ribose) polymerase family, member 1 antibody;Poly [ADP-ribose] polymerase 1 antibody;Poly(ADP ribose) polymerase antibody;poly(ADP ribose) synthetase antibody;poly(ADP ribosyl)transferase antibody;Poly[ADP ribose] synthetase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity.