PARP2 Recombinant Rabbit Monoclonal Antibody [JG34-56] (ET7108-05)
Catalog# ET7108-05
Rabbit Monoclonal to PARP2
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of PARP2 on different lysates with Rabbit anti-PARP2 antibody (ET7108-05) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.