ADAR Recombinant Rabbit Monoclonal Antibody [JU99-33] (ET7106-72)

UNIPROT #

P55265

PROTEIN NAME

ADAR

SYNONYMS

136 kDa double-stranded RNA-binding protein antibody;136kDa double stranded RNA binding protein antibody;Adar 1 antibody;ADAR antibody;Adar1 antibody;Adenosine deaminase acting on RNA 1 A antibody;Adenosine deaminase RNA specific 1 antibody;Adenosine deaminase RNA specific antibody;Adenosine deaminase that act on RNA antibody;AGS6 antibody;AV242451 antibody;Double stranded RNA specific adenosine deaminase antibody;Double-stranded RNA-specific adenosine deaminase antibody;Double-stranded RNA-specific editase Adar antibody;DRADA antibody;Dsh antibody;Dsrad antibody;DSRAD_HUMAN antibody;dsRNA adenosine deaminase antibody;EC 3.5.4.- antibody;G1P1 antibody;IFI 4 antibody;IFI-4 antibody;IFI4 antibody;Ifi4 protein antibody;Interferon induced protein 4 antibody;Interferon inducible protein 4 antibody;Interferon-inducible protein 4 antibody;K88DSRBP antibody;mZaADAR antibody;P136 antibody;Pre-mRNA adenosine deaminase antibody;RNA adenosine deaminase 1 antibody;RNA-editing deaminase 1 antibody;RNA-editing enzyme 1 antibody

TISSUE SPECIFICITY

Ubiquitously expressed, highest levels were found in brain and lung. Isoform 5 is expressed at higher levels in astrocytomas as compared to normal brain tissue and expression increases strikingly with the severity of the tumor, being higher in the most aggressive tumors.

POST-TRANSLATIONAL MODIFICATION

Sumoylation reduces RNA-editing activity.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells.