KDM5B Recombinant Rabbit Monoclonal Antibody [PSH03-97] (HA722076)
Catalog# HA722076
Rabbit Monoclonal to KDM5B
Tested for
-
WB
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (HA722076) at 1/2,000 dilution.
Lane 1: BT-20 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: AGS cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 176 kDa
Observed band size: 145 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722076) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
Lane 1: BT-20 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: AGS cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 176 kDa
Observed band size: 145 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722076) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.