PHF8 Rabbit Polyclonal Antibody (ER1803-37)

UNIPROT #

Q9UPP1

PROTEIN NAME

PHF8

SYNONYMS

Histone lysine demethylase PHF8 antibody;JHDM1F antibody;Jumonji C domain containing histone demethylase 1F antibody;MRXSSD antibody;PHD finger protein 8 antibody;PHF8 antibody;PHF8_HUMAN antibody;ZNF422 antibody

SEQUENCE SIMILARITIES

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.

SUBCELLULAR LOCATION

Nuclear membrane.

FUNCTION

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.