ASH2L Recombinant Rabbit Monoclonal Antibody [ST47-01] (ET1609-24)

UNIPROT #

Q9UBL3

PROTEIN NAME

ASH2L

SYNONYMS

ASH 2 antibody;Ash2 (absent small or homeotic) like antibody;Ash2 (absent, small, or homeotic) like (Drosophila) antibody;ASH2 antibody;ASH2 LIKE antibody;ASH2 like protein antibody;ASH2-like protein antibody;Ash2l antibody;ASH2L_HUMAN antibody;ASH2L1 antibody;ASH2L2 antibody;Bre 2 antibody;Bre2 antibody;Discs 2-like antibody;Drosophila absent, small, or homeotic antibody;Drosophila ASH2-like antibody;Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody

TISSUE SPECIFICITY

Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.

POST-TRANSLATIONAL MODIFICATION

Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Alternative splicing results in a shorter isoform 2, designated ASH2L2, which is missing the first 94 amino acid residues found in ASH2L1. Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. ASH2L is highly expressed in fetal liver, testis and leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cells lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia.