KDM3B Recombinant Rabbit Monoclonal Antibody [1A8] (HA721170)
Catalog# HA721170
Rabbit Monoclonal to KDM3B
Tested for
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WB
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IHC-P
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IF-Cell
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA721170) at 1/1,000 dilution.
Lane 1: Hela cell lysate (25 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 191 kDa
Observed band size: 191 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate (25 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Predicted band size: 191 kDa
Observed band size: 191 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.