PRMT7 Recombinant Rabbit Monoclonal Antibody [JE64-78] (HA721996)
Catalog# HA721996
Rabbit Monoclonal to PRMT7
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PRMT7 on different lysates with Rabbit anti-PRMT7 antibody (HA721996) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: HEK-293 cell lysate (15 µg/Lane)
Lane 5: 293T cell lysate (15 µg/Lane)
Lane 6: MCF7 cell lysate (15 µg/Lane)
Lane 7: NIH/3T3 cell lysate (15 µg/Lane)
Lane 8: Mouse testis tissue lysate (30 µg/Lane)
Lane 9: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721996) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: HEK-293 cell lysate (15 µg/Lane)
Lane 5: 293T cell lysate (15 µg/Lane)
Lane 6: MCF7 cell lysate (15 µg/Lane)
Lane 7: NIH/3T3 cell lysate (15 µg/Lane)
Lane 8: Mouse testis tissue lysate (30 µg/Lane)
Lane 9: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721996) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.