Thymidine Phosphorylase Mouse Monoclonal Antibody [A1A10] (EM1901-22)
Catalog# EM1901-22
Mouse Monoclonal to Thymidine Phosphorylase
Tested for
-
WB
-
IHC-P
-
FC
-
IF-Cell
Reacts with
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Human
-
Mouse
-
Rat
Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (EM1901-22) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: A549 cell lysate
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: A549 cell lysate
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.