NEDD4-2 Recombinant Rabbit Monoclonal Antibody [SN73-03] (ET1611-42)

UNIPROT #

Q96PU5

PROTEIN NAME

NEDD4-2

SYNONYMS

E3 ubiquitin protein ligase NEDD4 like protein antibody;E3 ubiquitin-protein ligase NEDD4-like antibody;KIAA0439 antibody;NED4L_HUMAN antibody;NEDD4 2 antibody;NEDD4 2c antibody;Nedd4-2 antibody;NEDD4-2a antibody;NEDD4.2 antibody;NEDD4b antibody;NEDD4L antibody;NEDD4La antibody;NEDD4Lb antibody;NEDD4Lc antibody;NEDD4Ld antibody;NEDD4Le antibody;NEDD4Lf antibody;NEDD4Lg antibody;NEDD4Lh antibody;NEDL3 antibody;Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody;Neural precursor cell expressed, developmentally down regulated 4 like antibody;RSP5 antibody;Ubiquitin protein ligase Rsp5 antibody

TISSUE SPECIFICITY

Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.; Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.

SUBCELLULAR LOCATION

Cytoplasm, Endosome, Golgi apparatus.

FUNCTION

NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGFβ RII, NEDD4-L is thought to inhibit the TGFβ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events.