SET Recombinant Rabbit Monoclonal Antibody [JB60-34] (ET7107-18)

UNIPROT #

Q01105

PROTEIN NAME

SET

SYNONYMS

2PP2A antibody;HLA DR associated protein II antibody;HLA-DR-associated protein II antibody;I 2PP2A antibody;I-2PP2A antibody;I2PP2A antibody;IGAAD antibody;Inhibitor of granzyme A activated DNase antibody;Inhibitor of granzyme A-activated DNase antibody;Inhibitor of GzmA-activated DNase antibody;inhibitor-2 of protein phosphatase-2A antibody;IPP2A2 antibody;PHAPII antibody;Phosphatase 2A inhibitor I2PP2A antibody;protein phosphatase type 2A inhibitor antibody;Protein SET antibody;Set antibody;SET nuclear oncogene antibody;SET translocation antibody;SET translocation (myeloid leukemia-associated) antibody;SET_HUMAN antibody;TAF I antibody;TAF IBETA antibody;TAF-I antibody;TAFI antibody;Template activating factor I antibody;Template-activating factor I antibody;Template-Activating Factor-I, chromatin remodelling factor antibody

SEQUENCE SIMILARITIES

Belongs to the nucleosome assembly protein (NAP) family.

TISSUE SPECIFICITY

Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

POST-TRANSLATIONAL MODIFICATION

Isoform 2 is phosphorylated on Ser-15 and Ser-24.; Isoform 2 is acetylated on Lys-11.; Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).; N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity).; [Isoform 2]: Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Endoplasmic reticulum.

FUNCTION

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.