BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R] (HA601345)
Catalog# HA601345
Mouse Monoclonal to BRG1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of BRG1 on different lysates with Mouse anti-BRG1 antibody (HA601345) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A549 cell lysate (negative)
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: A549 cell lysate (negative)
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.