CHD1 Mouse Monoclonal Antibody [2-2] (M1211-5)
Catalog# M1211-5
Mouse Monoclonal to CHD1
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
-
Human
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CHD1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 260 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CHD1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 260 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.