SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15] (HA721627)
Catalog# HA721627
Rabbit Monoclonal to SMARCA2
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (HA721627) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NCCIT cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721627) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NCCIT cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721627) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.