SMARCA2 Rabbit Polyclonal Antibody (ER65406)
Catalog# ER65406
Rabbit Polyclonal to SMARCA2
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (ER65406) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65406) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65406) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
