KIF4A Recombinant Rabbit Monoclonal Antibody [JE38-91] (HA721591)
Catalog# HA721591
Rabbit Monoclonal to KIF4A
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KIF4A on different lysates with Rabbit anti-KIF4A antibody (HA721591) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 160 kDa
Exposure time: 6 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 160 kDa
Exposure time: 6 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721591) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.