DNMT1 Mouse Monoclonal Antibody [A3A8] (EM1901-83)

UNIPROT #

P26358

PROTEIN NAME

DNMT1

SYNONYMS

ADCADN antibody;AIM antibody;CXXC finger protein 9 antibody;CXXC-type zinc finger protein 9 antibody;CXXC9 antibody;DNA (cytosine 5 ) methyltransferase 1 antibody;DNA (cytosine-5)-methyltransferase 1 antibody;DNA methyltransferase 1 antibody;DNA methyltransferase HsaI antibody;DNA methyltransferase M.HsaI. antibody;DNA MTase antibody;DNA MTase HsaI antibody;DNMT 1 antibody;DNMT antibody;Dnmt1 antibody;DNMT1_HUMAN antibody;Dnmt1o antibody;FLJ16293 antibody;HSN1E antibody;M.HsaI antibody;MCMT antibody;Met1 antibody;MGC104992 antibody;mMmul antibody;MommeD2 antibody

SEQUENCE SIMILARITIES

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

TISSUE SPECIFICITY

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

POST-TRANSLATIONAL MODIFICATION

Sumoylated; sumoylation increases activity.; Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.; Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.; Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.; Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.