MGMT Rabbit Polyclonal Antibody (ER40104)
Catalog# ER40104
Rabbit Polyclonal to MGMT
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of MGMT on different lysates with Rabbit anti-MGMT antibody (ER40104) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-MGMT KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40104) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-MGMT KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40104) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
