Histone H1.0 Recombinant Rabbit Monoclonal Antibody [SD206-04] (ET1612-24)
Catalog# ET1612-24
Rabbit Monoclonal to Histone H1.0
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Histone H1.0 on different lysates with Rabbit anti-Histone H1.0 antibody (ET1612-24) at 1/500 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.