Phospho-Estrogen Receptor alpha (S118) Recombinant Rabbit Monoclonal Antibody [SC05-87] (ET1610-32)

UNIPROT #

P03372

PROTEIN NAME

Phospho-Estrogen Receptor alpha (S118)

SYNONYMS

DKFZp686N23123 antibody;ER alpha antibody;ER antibody;ER-alpha antibody;Era antibody;ESR antibody;ESR1 antibody;ESR1_HUMAN antibody;ESRA antibody;Estradiol receptor antibody;Estrogen nuclear receptor alpha antibody;Estrogen receptor 1 antibody;Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody;Estrogen receptor alpha antibody;Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody;Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody;Estrogen receptor alpha delta 4 +49 isoform antibody;Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody;Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody;Estrogen receptor alpha E1 E2 1 2 antibody;Estrogen receptor alpha E1 N2 E2 1 2 antibody;Estrogen receptor antibody;ESTRR antibody;NR3A1 antibody;Nuclear receptor subfamily 3 group A member 1 antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR3 subfamily.

TISSUE SPECIFICITY

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro.; Glycosylated; contains N-acetylglucosamine, probably O-linked.; Ubiquitinated; regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1.; Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260.; Palmitoylated (isoform 3). Not biotinylated (isoform 3).; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Cell membrane.

FUNCTION

Estrogen receptor alpha (ERa, ER, ESR, ESRA, Era, NR3A1, estrogen receptor 1) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. Alternative splicing results in several ERa mRNA transcripts, which differ primarily in their 5' untranslated regions. ERa undergoes phosphorylation in response to estradiol binding. Human ERa is predominately phosphorylated on Ser 118 and to a lesser extent on Ser 104 and Ser 106. In response to activation of the mitogen-activated protein kinase pathway, phosphorylation occurs on Ser 118 and Ser 167. These serine residues are all located within the activation function 1 region of the N-terminal domain of ERa. In contrast, activation of protein kinase A increases the phosphorylation of Ser 236, which is located in the DNA-binding domain. Src kinase-dependent Tyr 537 phosphorylation may enhance estrogen binding to ERa. Mutation of Tyr 537 of the human ERa produces receptors having a range of constitutive activity.