Pluripotency Transcription Factor Antibody Kit (HAK21089)
Catalog# HAK21089
Reacts with
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Human
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Mouse
Western blot analysis of Nanog on different lysates with Rabbit anti-Nanog antibody (ET1610-2) at 1/2,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NCCIT cell lysate
Lane 2: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.