Stat Antibody Sampler Kit (HAK21013)
Catalog# HAK21013
Reacts with
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Human
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Mouse
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Rat
Western blot analysis of STAT1 on different lysates with Rabbit anti-STAT1 antibody (ET1606-39) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: A431 cell lysate (10 µg/Lane)
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-39) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: A431 cell lysate (10 µg/Lane)
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-39) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.