LIN28A Mouse Monoclonal Antibody [A4-A6] (M1301-1)
Catalog# M1301-1
Mouse Monoclonal to LIN28A
Tested for
-
WB
-
IF-Cell
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IHC-P
-
FC
Reacts with
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Human
-
Mouse
Western blot analysis of Lin28A on different lysates with Mouse anti-Lin28A antibody (M1301-1) at 1/1,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: JAR cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa
Exposure time: Lane 1-2: 9 seconds; Lane 3: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1301-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NCCIT cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: JAR cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa
Exposure time: Lane 1-2: 9 seconds; Lane 3: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1301-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.