Lin28A Recombinant Rabbit Monoclonal Antibody [JE60-36] (HA722899)
Catalog# HA722899
Rabbit Monoclonal to Lin28A
Tested for
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WB
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IF-Cell
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FC
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IP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Lin28A on different lysates with Rabbit anti-Lin28A antibody (HA722899) at 1/1,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: JAR cell lysate
Lane 3: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 29 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722899) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NCCIT cell lysate
Lane 2: JAR cell lysate
Lane 3: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 29 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722899) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.