Argonaute 2 Recombinant Rabbit Monoclonal Antibody [JF0992] (ET1702-39)
Catalog# ET1702-39
Rabbit Monoclonal to Argonaute 2
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
All lanes: Western blot analysis of Ago2 with anti-Ago2 antibody [JF0992] (ET1702-39) at 1:500 dilution.
Lane 1: Wild-type CHO whole cell lysate.
Lane 2: Ago2 knockout CHO whole cell lysate.
ET1702-39 was shown to specifically react with Ago2 in wild-type CHO cells. No band was observed when Ago2 knockout samples were tested. Wild-type and Ago2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Ago2 antibody (ET1702-39, 1/500) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Lane 1: Wild-type CHO whole cell lysate.
Lane 2: Ago2 knockout CHO whole cell lysate.
ET1702-39 was shown to specifically react with Ago2 in wild-type CHO cells. No band was observed when Ago2 knockout samples were tested. Wild-type and Ago2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Ago2 antibody (ET1702-39, 1/500) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
