eIF4G1 Recombinant Rabbit Monoclonal Antibody [PSH06-28] (HA722660)
Catalog# HA722660
Rabbit Monoclonal to eIF4G1
Tested for
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WB
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IHC-P
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IF-Cell
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of eIF4G1 on different lysates with Rabbit anti-eIF4G1 antibody (HA722660) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: PANC-1 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A431 cell lysate
Lane 4: 293T cell lysate
Lane 5: COS-1 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 175 kDa
Observed band size: 250 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722660) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: PANC-1 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A431 cell lysate
Lane 4: 293T cell lysate
Lane 5: COS-1 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 175 kDa
Observed band size: 250 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722660) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.