USP7 Recombinant Rabbit Monoclonal Antibody [JB80-36] (ET7107-53)
Catalog# ET7107-53
Rabbit Monoclonal to USP7
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
All lanes: Western blot analysis of USP7 with anti-USP7 antibody [JB80-36] (ET7107-53) at 1:1,000 dilution.
Lane 1: Wild-type A375 whole cell lysate (20 µg).
Lane 2: USP7 knockout A375 whole cell lysate (20 µg).
ET7107-53 was shown to specifically react with USP7 in wild-type A375 cells. No band was observed when USP7 knockout sample was tested. Wild-type and USP7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-53, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Wild-type A375 whole cell lysate (20 µg).
Lane 2: USP7 knockout A375 whole cell lysate (20 µg).
ET7107-53 was shown to specifically react with USP7 in wild-type A375 cells. No band was observed when USP7 knockout sample was tested. Wild-type and USP7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7107-53, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
