USP9x Recombinant Rabbit Monoclonal Antibody [JG35-11] (ET7108-08)
Catalog# ET7108-08
Rabbit Monoclonal to USP9x
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of USP9x on different lysates with Rabbit anti-USP9x antibody (ET7108-08) at 1/500 dilution.
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lane 3: 293 cell lysate
Lane 4: Mouse colon tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 290 kDa
Observed band size: 290 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lane 3: 293 cell lysate
Lane 4: Mouse colon tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 290 kDa
Observed band size: 290 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.