TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH10-00] (HA723169)
Catalog# HA723169
Rabbit Monoclonal to TIM 3
Tested for
-
WB
-
IF-Cell
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FC
-
IP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA723169) at 1/2,000 dilution.
Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (negative) (20 µg/Lane)
Lane 3: HUT 102 cell lysate (20 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723169) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (negative) (20 µg/Lane)
Lane 3: HUT 102 cell lysate (20 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723169) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.