IFNAR1 Mouse Monoclonal Antibody [A5-A3] (EM1701-46)
Catalog# EM1701-46
Mouse Monoclonal to IFNAR1
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
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Human
-
Mouse
Western blot analysis of IFNAR1 on different lysates with Mouse anti-IFNAR1 antibody (EM1701-46) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64/90/130 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-46) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64/90/130 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-46) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.