CD147 Recombinant Rabbit Monoclonal Antibody [JF1-045] (ET1702-58)
Catalog# ET1702-58
Rabbit Monoclonal to CD147
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of CD147 on different lysates with Rabbit anti-CD147 antibody (ET1702-58) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: NCI-H226 cell lysate (15 µg/Lane)
Lane 3: 786-0 cell lysate (15 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse liver tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 45-60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: NCI-H226 cell lysate (15 µg/Lane)
Lane 3: 786-0 cell lysate (15 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse liver tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 45-60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.